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Based on general GLP-1 timelines , anti-inflammatory effects likely begin within weeks, but meaningful symptom changes may take 2 to 3 months as both direct effects and weight-mediated estrogen reduction accumulate

[11] Copper overload Because systemic GHK-Cu is functionally a route of copper delivery, repeated or high systemic exposure raises a legitimate concern about copper accumulation

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Therefore, anything that causes temporary or permanent damage to any of these organs could potentially affect DAO function, particularly damage to the intestines, because that is where DAO is most active (in non-pregnant individuals)